Fig. 5 | Scientific Reports

Fig. 5

From: Characterization of a phosphoinositide-binding protein containing a PHOX homology domain in the malaria parasite Plasmodium falciparum

Fig. 5

Identification of PfPX2 proximal proteins by Di-BioID. (A) Illustration of the Di-BioID principle. (B) Live microscopy demonstrating that incubation of the PfPX2-2xFKBP-GFP + FRB-BirA-mCherry parasite line with 250 nM rapamycin for 24 h results in the translocation of the mCherry signal to PfPX2-2xFKBP-GFP foci. Scale bar = 5 μm. Blue: DAPI-stained nuclei. (C) Immunofluorescence analysis with anti-streptavidin confirmed successful biotinylation at the PfPX2-2xFKBP-GFP foci (D) Heat map showing proteins with ≥ 1.5-fold enrichment across three biological replicates. Data expressed as log2. Arrows indicate PfPX2, PfSortilin, VPS13, the clathrin heavy chain, GRIP and coiled-coil domain-containing protein and PfDyn1.

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