Table 1 Primer sequences for the target genes.
From: mTOR/HIF-1α-associated scleral metabolic reprogramming by Mingshi formula in form-deprivation myopia
Gene | Primer sequences |
|---|---|
a-SMA | Forward: 5′-AAGAGCATCCAACCCTGCTC-3′ |
Reverse: 5′-AGAGGCATAGAGGGACAGCA-3′ | |
MMP2 | Forward: 5′-GTGAAGTACGGGAATGCCGA-3′ |
Reverse: 5′-CGTCTGTGCAGCTGGTGTAT-3′ | |
MTOR | Forward: 5′-GCGTTTGTGTCCTGTTGGTC-3′ |
Reverse: 5′-TGCTCCATGAACTCAGCCAG-3′ | |
Collagen 1 | Forward: 5′-TTCAGCTTTGTGGACCTCCG-3′ |
Reverse: 5′-TTTCCAGGTGTCTCCGTTGG-3′ | |
Hif1a | Forward: 5′-AGGATAAGTTCTGAACGTCGAAAAG-3′ |
Reverse: 5′-ACATTGTGGGGAAGTGGCAA-3′ | |
Gapdh | Forward: 5′-TTCTACCCACGGCAAGTTCC-3′ |
Reverse: 5′-CCAGCATCACCCCACTTGAT-3′ |
