Fig. 3

Characterisation of the endoscope biofilm model (EBM). Biofilm densities (log10 CFU cm- 2) of Pseudomonas aeruginosa (green filled circle), Staphylococcus aureus (orange filled square), Klebsiella pneumoniae (purple upside down filled triangle) and Escherichia coli (yellow filled traingle) after 72 h culture in the EBM with a flow rate of 1.25 mL min- 1 (n = 3 ± SD). Sections cut from 50 cm length of PTFE tubing used within the EBM. In the direction of flow, first 5 cm discarded, Sect. 1 = 5 cm to 15 cm, Sect. 2 = 15 cm to 25 cm, Sect. 3 = 25 cm to 35 cm, Sect. 4 = 35 to 45 cm, last 5 cm discarded. Within each section, three 1 cm subsections were processed for biofilm recovery.