Table 1 Primers designed for this study.
Gene | Sequence | Tm | Amplicon (bp) |
|---|---|---|---|
ALG9 | 3’-CCATCTCAGGATCCCTCTTC-5’ | 53.8 | 148 |
5’-CGATTCCAGCGAATAGTTGA-3’ | 51.7 | ||
UBC6 | 3’-CCATACGAGAATGGACAATACCACG-5’ | 57.7 | 172 |
5’-CCACAGACCATGCTGGGTTCC-3’ | 58.3 | ||
ARO3 | 3’-TCTGCGGTGTAATGATCGAGTC-5’ | 54.8 | 133 |
5’-ACCTCATCAGTGGTATCCCAA-3’ | 52.4 | ||
ARO4 | 3’-CTGGTTTATCCTTCCCAGTCG-5’ | 54.4 | 191 |
5’-TGTTCCCTTCTTACCACCTC-3’ | 51.8 | ||
PHA2 | 3’-ATTGGAAGTCGTGCTAGTGC-5’ | 58.4 | 120 |
5’-GCGATTATTCGGCTTACGCCTTC-3’ | 57.1 | ||
ARO8 | 3’-AATTCGGACAACCACAGAGCA-5’ | 52.4 | 186 |
5’-GCCCTATAACCCAGCCTAATC-3’ | 54.4 | ||
ARO9 | 3’-GACGGTACGATCACCTATGAC-5’ | 54.4 | 63 |
5’-AATTGTGAGGTAGGATGGCGT-3’ | 52.4 | ||
ARO10 | 3’-GATCTTGTGTGAAGGTGATGGTG-5’ | 55.3 | 141 |
5’-CTGGTTGGTCCTAGAATAGC-3’ | 51.8 | ||
ARO80 | 3’-GCTCTCTGTACTATCACCGTTG-5’ | 54.8 | 294 |
5’-TTCGCAGCGTTGTATGCAAG-3’ | 51.8 | ||
CAT8 | 3’-CGCTCGTGGTTCGTTAGAGTAC-5’ | 56.7 | 132 |
5’-CTATGCCAGCTCATTGTGC-3’ | 57.5 | ||
MIG1 | 3’-GCTCAAACATGGGATTCGGAACC-5’ | 57.1 | 120 |
5’-CCTTCTCATCACCAGCACTACG-3’ | 56.7 |
