Fig. 4
From: Revealing neural resonance in neuronal ensembles through frequency response tests
Frequency responses of extracellular spiking activity and LFP in CA1. (a) In vivo extracellular recordings was performed while mice were subjected to pulsed tNIR stimulation, allowing them to move freely on a rotatable plate. (b) An example of trial-by-trial raster plot locked to the time of tNIR-stimulation for one example multi-unit. (c) Top, waveforms of the action potentials recorded extracellularly. Bottom, trial-averaged peri-event time histogram. (d) Compares the pre-stimulation and during-stimulation FR at different tNIR frequencies (****p < 0.0001, n = 8 mice, two-sided paired t-test). e Right, the frequency-response curve shows the net FR change (one-way ANOVA, F(19, 133) = 9.5591, P = 6.55 × 10−17). Left, Tukey’s test identified significant group differences, highlighted by red dots. Dots and error bars represent means and 95% confidence intervals, respectively. (f) The red line represents the LFP power spectral density during stimulation, while the blue line indicates the LFP power spectral density prior to stimulation. n = 8 mice. (g) The signal coefficient of variance (solid line) and the noise coefficient of variance (dashed line) of LFP as function of frequency, with red and blue lines representing during and prior to stimulation, respectively. n = 8 mice. (h) Comparison of pre-stimulation and during-stimulation power (110–120 Hz spectral frequency band) at different tNIR frequencies (****p < 0.0001, n = 8 mice, two-sided paired t-test). (i) Right, the frequency-response curvy for the net change in power (F(19, 133) = 16.1017, P = 7.56 × 10−26, mean ± s.e.m). Left, Tukey’s test was applied to the data (right) to identify precisely which group means differed. Significance peaks among the groups are highlighted with red dots. Dots and error bars represent means and 95% confidence intervals, respectively. mean ± s.e.m.
