Fig. 4
Changes in microRNA (miRNA) expression in total plasma and plasma-derived exosomes of patients with medication-related osteonecrosis of the jaw (MRONJ) compared to healthy controls. (A) Bar plots depicting the effect size, log-scaled fold change (logFC), and statistical significance of miRNA changes in MRONJ patients across two sample types (total plasma vs. plasma exosome). Among the five miRNAs that showed significant (q < 0.05) dysregulation in total plasma samples from the MRONJ group, miR-483-5p, miR-92b-5p, miR-628-3p, and miR-486-5p exhibited consistent directional changes in plasma-derived exosomes. logFC values were calculated by subtracting the mean log-scaled calibrated normalized relative quantity (CNRQ) value of the control group from that of the MRONJ group, and the values from each sample type were compared for each miRNA. (B) Predicted log-scaled CNRQ values for subject groups (Control [CON] vs. MRONJ) and sample types (total plasma vs. plasma exosome) for the four miRNAs, estimated from linear mixed-effects models. The fitted lines represent the interaction between subject group and sample type, with each panel displaying a different miRNA target. The interaction term for each miRNA was not statistically significant (NS), indicating consistent dysregulation across sample types.
