Fig. 4

Fig 4.1 Spleen tissue of rats across different groups: (a) In the control group, the white pulp is clearly defined with its three compartments: periarteriolar lymphoid sheath, lymphoid follicles, and marginal zone. (b) The IS group shows white pulp atrophy and lymphocyte depletion. (c) The CS group exhibits repopulation of the white pulp (d) C.CS group, (e) Iv group, (f) Iv-CS group, (g) Iv-C.CS group, (h) Sc group, (i) Sc-CS group, (j) Sc- C.CS group, (k) Gg group, (l) Gg-CS group, (m) Gg-C.CS group. (Hematoxylin and eosin stain X 200).Fig 4.2 Ki-67 immunohistochemistry in splenic tissue of rats in different groups. (a) Weak expression of Ki-67 in the white pulp of control group and (b) IS group. (c) the expression of Ki-67 was observed in the lymphocytes populating the white pulp in CS group, (d) C.CS group, (e) Iv group, (f) Iv-CS group, (g) Iv-C.CS group, (h) Sc group, (i) Sc-CS group, (J) Sc-C.CS group, (k) Gg group, (l) Gg-CS, (m) Gg-C.CS group. (Immunoperoxidase X400).Fig 4.3Ki-67 immunohistochemistry area percentages in splenic tissue of different rat groups. The columns indicate mean area percentages, with thin lines representing standard errors. Columns with different lowercase letters denote significant differences at p < 0.05.