Fig. 3 | Scientific Reports

Fig. 3

From: Alfalfa MsSOS2 confers salinity tolerance by promoting lateral root growth and regulating Na+/K+ homeostasis

Fig. 3

MsSOS2 interacts with MsSOS3 in yeast. Pairwise protein–protein interactions were performed between the specified proteins employing the yeast two-hybrid assay. Transformed yeast clones containing both pGBKT7 and pGADT7 constructs were examined in three tenfold serial dilutions (OD 600 = 0.1, 0.01, and 0.001) on double dropout (DDO) media, SD/-Leu/-Trp. The interaction status of MsSOS2 with MsSOS3, evaluated by the growth assay of yeast cells on interaction-selective Quadruple Dropout media containing X-α-Gal and Aureobasidin (XDO/X/A), SD/-Leu/-Trp/-His/X/A. Empty vectors (EV) were transformed to check the auto-activation status. The Lam and T-antigen protein pair was used as a negative control, while the P53 and T-antigen protein pair was used as positive control.

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