Fig. 5
Expression status of the MsSOS2 gene in Col-0, sos2 mutant, MsSOS2-4-7, and MsSOS2-9-7 plants by RT-qPCR. Five leaves were used per replicate, and three biological replicates were used in the assay (n = 3). The y-axis shows relative normalized expression of MsSOS2, while the x-axis indicates the expression levels of MsSOS2 in the leaves of the indicated genotypes. Error bars represent the standard error (± SE). For statistical analysis, a two-way ANOVA and a Tukey’s HSD test were used to show statistical differences in MsSOS2 expression between indicated genotypes, and different letters above the error bars indicate a significant difference (P < 0.05), while the same letters indicate no significant difference.
