Fig. 6

MsSOS2 positively regulates seed germination in response to salinity. Fifty seeds were used per replicate, and three biological replicates were used in the assay (n = 3). (a) Seed germination in a control environment on a ½ MS-Agar plate. (b) Seed germination on ½ MS-Agar plates containing 50 mM NaCl. (c) Seed germination on ½ MS-Agar plates containing 75 mM NaCl. (d) Relative percentage of seed germination for the indicated genotypes under 50 mM NaCl and (e) Relative percentage of seed germination for the indicated genotypes under 75 mM NaCl. Error bars represent the standard error (± SE). For statistical analysis (d,e), a two-way ANOVA and a Tukey’s HSD test were used to show statistical differences in relative germination % of seeds between indicated genotypes (d,e), different letters above the error bars indicate a significant difference (P < 0.05), while the same letters indicate no significant difference.