Fig. 7
Effect of salinity on seedling growth and lateral root development. Three seedlings of the indicated genotypes were grown per replicate on control or 60 mM NaCl media to quantify lateral root numbers and net primary root length, with five biological replicates used in the assay (n = 5). (a) Seedling growth of the indicated genotypes (Col-0, sos2 mutant, and two homozygous MsSOS2 transgenic lines: 4–7 and 9–7) is shown on control ½ MS-Agar media. (b) Seedling growth on ½ MS-Agar media containing 60 mM NaCl. (c) The number of lateral roots per plant for each genotype, grown on control media (blue bars) and media containing 60 mM NaCl (orange bars), is also presented. Error bars represent the standard error (± SE). For statistical analysis, a two-way ANOVA followed by a Tukey’s HSD test was used to determine significant differences in lateral root numbers (c) and net primary root length (d) between and among the indicated genotypes. In panels (c) and (d), different letters above the error bars indicate a significant difference (P < 0.05), whereas the same letters indicate no significant difference.
