Fig. 3
From: Elucidating the activation mechanism of botulinum neurotoxin a: role of α-clostripain and NTNH
Evaluation of the activity of α-clostripain on LCHN and BoNT/Ai. (A) Activity assay with rα-clostripain and C. botulinum A culture supernatant. Lane 1–MW marker; lane 2–LCHN; lane 3—C. botulinum A culture supernatant; lane 4–rα-clostripain; lane 5–activity assay of C. botulinum A culture supernatant; lane 6–activity assay of rα-clostripain. (B) Evaluation of the activity of rα-clostripain on rBoNT/Ai. Lane 1–MW marker; lane 2–rBoNT/Ai; lane 3–rα-clostripain; lane 4–rBoNT/Ai + rα-clostripain; lane 5—C. botulinum A culture supernatant; lane 6—rBoNT/Ai + C. botulinum A culture supernatant. rα-clostripain and C. botulinum A culture supernatant cleave rBoNT/Ai non-specifically.
