Fig. 5 | Scientific Reports

Fig. 5

From: Electron fourier ptychography for phase reconstruction

Fig. 5

Exit wave reconstruction of Cry11Aa and rotavirus samples. (a1)-(a4) Reconstructed amplitude, phase, and calculated power spectra of the experimental Cry11Aa data. Insets to (a2) and (a3) shows the reconstructed amplitude and phase from simulated Cry11Aa data using the experimental parameters. The upper section of (a4) shows the power spectrum of the reconstructed phase, while the lower section displays the power spectrum of the reconstructed amplitude. Inset to (a4) is an enlarged view of the reflection at the resolution of 0.63 nm in the power spectrum of reconstructed phase. (b1)-(b3) Reconstructed amplitude and phase of rotavirus particles. (b4) Plots of circular averages of the amplitude of the Fourier transform of the reconstructed phase (red) and amplitude (black), respectively. Plots are normalised with respect to the intensities of peaks at 0.12 nm−1, corresponding to the separation between the capsid trimers of viral protein 6 (VP6)17. The phase scale bars in images (a3) and (b3) are in radians. The reconstructed amplitude and phase in (a1)-(a3) and (b1)-(b3) are filtered using Gaussian filters with standard deviations of 2 and 3, respectively.

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