Table 1 Parameters for eFP data simulations. The notation of the aberration parameters follows that given by Saxton52. Positive \(\:{C}_{1}\) values are defined as overfocus.
From: Electron fourier ptychography for phase reconstruction
Gold particle | Apoferritin | Cry11Aa | |
|---|---|---|---|
Simulation box size (nm3) | 13.5 × 13.5 × 0.8 | 102.4 × 102.4 × 30.0 | 153.6 × 153.6 × 30 |
Slice thickness (nm) | 0.14 | 1.00 | 1.00 |
Number of slices | 6 | 31 | 31 |
Accelerating voltage (kV) | 300 | 300 | 300 |
Illumination semiangle (mrad) | 0.02 | 0.02 | 0.02 |
Focal spread (nm) | 4.3 | 8.5 | 8.5 |
\(\:{C}_{1}\) (nm) | 2.5 | −2000 | −2172 |
\(\:{C}_{3}\) (mm) | −1.9 × 10−5 | 2.7 | 2.7 |
Pixel size (nm/pixel) | 0.013 | 0.05 | 0.25 |
Tilt magnitudes (mrad) | 10 and 15 | 5 | 1.9 |
Total fluence (e−/nm2) | 4.6 × 105 | 1 × 103, 3 × 103, 9 × 103, and infinite fluence | 4.5 × 102 |
Number of tilts | 7 | 4, 5, 9, and 13 | 4 |
