Fig. 4 | Scientific Reports

Fig. 4

From: Dictyostelium exhibits PCB-induced impairment of proliferation, development and stress response, emerging as model for conserved environmental toxicity

Fig. 4

Expression of iron-related and cAMP signaling pathways genes following PCB treatments and assessment of intracellular iron levels following PCB exposure. Ax4 cells were treated for 3 days with PCB 138 and PCB 153 (50 µM) before the analysis. The relative expression of iron-related (A) and developmental (C) genes are reported in the graphs. Intracellular iron level was indirectly measured through calcein fluorescence quenching assay (B). Flow cytometry profiles (left panel) and mean fluorescence intensity (MFI) ratio quantification (right panel) are shown. Vehicle sample was set as control (= 1). Data are presented as mean values from three independent biological replicates. Statistical significance was determined for both experiments using One-way ANOVA with Dunnett’s multiple comparisons test (* = p < 0.05, ** = p < 0.01, *** = p < 0.001). This graph was generated with BioRender.

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