Table 1 Various decellularization methods for tilapia fish skin from the literature.
From: In vivo subcutaneous biocompatibility evaluation of decellularized tilapia fish skin in a rat model
Author(s) | Treatment method | Residual DNA content | References | |
|---|---|---|---|---|
1 | Lau et al. | SDS + Nuclease enzymes | ~ 99.6% DNA removed (~ 1.8 ± 0.9 ng/mg) | |
2 | Liu et al. | SDS + enzymatic treatment (for tendon scaffolds) | Significant reduction (dsDNA assay); exact value not given | |
3 | Esmaeili et al. | NaOH + Triton X-100 + Freeze–thaw cycles | 1.4 ± 0.7 ng/mg dry weight | |
4 | Huang et al. | NaOH (0.5 M) + SDS (1%) + Nuclease | 38.78 ± 5.84 ng/mg dry weight | |
5 | Ibrahim et al. | Chemical sterilization of tilapia skin xenograft | < 50 ng/mg (qualitative, sufficient for medical use) | |
6 | Garrity et al. | Tilapia skin hydrogel (decellularized, exact method not specified) | Not reported | |
7 | Luo et al. | Gelatin hydrogel from tilapia + stromal vascular fraction | Not reported | |
8 | Zhang et al. | 0.5% Triton X-100 + 1% SDS (ionic detergents) | 2.84% of native DNA remained | |
9 | Huang et al. | Fresh tilapia skin + chemical decellularization | 32.94 ng/mg dry weight | |
10 | We et al. | Hypertonic/hypotonic cycles + Triton X-100 | Qualitative only – “minimal genetic material” |
