Table 1 Primer sequences used to assess gene expression.
From: Toxic effects of biodegradable polylactic acid nanoplastics on developing zebrafish (Danio rerio)
Gene | Forward primer (5′ → 3′) | Reverse primer (5′ → 3′) | Accession number |
|---|---|---|---|
hmox1a | TGGACAGAAACGCAGACCAC | GACTGCTCTTGCCAATCTCTCT | NM_001127516.1 |
sod2 | CAAGGGACCACAGGTCTCATC | CTCGCTGACATTCTCCCAGT | NM_199976.1 |
sod1 | GAACAAGGCCGTTTGTGTGC | ACTGGCTTCTTTTCACCCTCT | NM_131294.1 |
il1β | GTAACCTGTACCTGGCCTGC | AGACCCGCTGATCTCCTTGAG | NM_212844.2 |
il4 | GCAGCATATACCGGGACTGG | ATGGCAGCATGCTTTGGTTT | NM_001170740.1 |
infγ1 | TGGGCGATCAAGGAAAACGA | TTGATGCTTTAGCCTGCCGT | NM_212864.1 |
nos2a | AACTCTCTACAGCGGCGGAT | ATTCCTGGAAGTCCGATGCG | NM_001104937.1 |
tbx21 | CGGGACAATTATGACACGCTG | AAGCGACTTTGAGGAAGGGG | NM_001170599.2 |
il13 | CCCCAAAAGAGACAAAGGCAA | TCACACTTCAGGCCACTTCC | NM_001199905.1 |
tnfα | AGGAGAGTTGCCTTTACCGC | GTGAGTCTCAGCACACTTCCA | NM_212859.2 |
gapdh | ATACACGGAGCACCAGGTTG | AATACCAGCACCAGCGTCAA | NM_001115114.1 |
