Fig. 4
Anterograde labeling of the corticospinal tract by injection of dextran amine into hindlimb motor cortex. (A) Furthest caudal dextran amine-traced axons. Each point represents the distance (mm) between the injury epicenter and the furthest caudal DA-traced axon in one animal. Bar represents the mean ± s.e.m of each group (One-way ANOVA; P = 0.0903, n = 4 PLO animals, n = 4 ASP animals, n = 3 No Tx animals). (B) Hoescht (cyan) stained cross section of spinal cord at T6 (rostral to the injury) confirms presence of dextran amine (yellow) in corticospinal tract (Scale bar 300 μm). (C) Hoescht (cyan) stained sagittal section of spinal cord from SCI animal with no scaffold. DA-traced axons (yellow) can be seen in the corticospinal tract (rostral aspect on the left, scale bar 500 μm). (D) Hoescht (cyan) stained sagittal section of spinal cord from SCI animal implanted with uncoated lignocellulosic scaffold (outlined by white dotted line). DA-traced axons (yellow) can be seen in the corticospinal tract (rostral aspect on the left, scale bar 500 μm). (E) Hoescht (cyan) stained sagittal section of spinal cord from SCI animal implanted with PLO-coated lignocellulosic scaffold (outlined by white dotted line). DA-traced axons (yellow) can be seen in the corticospinal tract (rostral aspect on the left, scale bar 500 μm).
