Fig. 1 | Scientific Reports

Fig. 1

From: Production system establishment and in vitro study of CD19/CD3ε bite antibody secreted from Pichia pastoris

Fig. 1

An in-house p-blinatumomab construction from Pichia pastoris expression system to produce blinatumomab. (a) The blinatumomab sequence was inserted in XhoI and BamHI enzyme cleavage sites of pHIL-S1 plasmid vector. p-blinatumomab BiTE antibody sequence transcript was added under the control of the AOX1 promotor with hexa-histidine (6×His) tag. Arginine was added at the front due to the restriction enzyme site. (b) Schematic representation of p-blinatumomab domain structure.

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