Fig. 3
Optimization of GS115/p-blinatumomab #105-produced p-blinatumomab expression. (a–c) Cultivation at 15, 30, and 37 ℃ for 6 days (144 h). P-blinatumomab was monitored by 12% SDS–PAGE gel analysis. The bar graph numerically represents the expression level for each day using a UV-Vis spectrophotometer. (d) The p-blinatumomab concentration after growth at 15 ℃ for 96 h in the presence of different MeOH concentrations (0, 0.5, 1.0, 2.0 and 4.0%). P-blinatumomab was monitored by 12% SDS–PAGE gel analysis. The bar graph numerically represents the daily expression level of p-blinatumomab, quantified by UV-Vis spectrophotometry at 280 nm. All values are expressed as the mean ± standard deviation (SD) based on three independent experiments (n = 3).
