Table 2 Different expression systems.

From: Production system establishment and in vitro study of CD19/CD3ε bite antibody secreted from Pichia pastoris

 

P. pastoris

E. coli

Mammalian cells

Secretion

++

-

+/++

Protein Productivity

++

+

-

Cost

--

--

++

Cost of growth medium

-

-

++ (CHO cells)

Doubling time

60–120 min

30 min

24 h (CHO cells)

  1. Table format and data partially adapted from Gonçalves et al. (2013) and Karbalaei, M. et al. (2020) with modification.
  2. ++ high, +/++ medium to high, + medium, - low; -- very low/absent.
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