Fig. 1 | Scientific Reports

Fig. 1

From: SIRT1/PGC-1α/Mfn2 pathway regulates mitochondrial homeostasis in VSMC to attenuate aging-related vascular calcification

Fig. 1

Mitochondrial homeostasis and Calcification on the Aging Process of Vascular Smooth Muscle. (A). SA-β-gal staining results demonstrate increased β-galactosidase activity in aged vascular smooth muscle cells compared to controls. (B). Calcium nodule detection shows a trend towards increased calcification in aged VSMCs. (C). Mito Tracker Red staining reveals reduced mitochondrial membrane potential in aged VSMCs. (D). Mito Tracker Green staining reveals mitochondrial morphological changes in aged VSMCs. (E). Western blot analysis shows decreased expression levels of SIRT1, PGC-1α, and Mfn2 in aged VSMCs compared to controls. ***P < 0.001, ****P < 0.0001, indicated a significant difference between groups.

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