Fig. 5
From: STAT1 and m6A-mediated IL15RA upregulation promotes metastasis via ZEB1/NF-κΒ axis in ccRCC
The role of IL15RA in genetic and epigenetic regulation, cell migration of ccRCC. (A-B). Western blotting results of IL15RA in cell lines and quantification statistical bar graph. (C). Dual luciferase assay indicated that STAT1 modulates IL15RA transcription activity in ACHN cell line. (D). Overexpression of METTL3 upregulates IL15RA expression in OSRC-2 and ACHN cell lines. (E-F). qRT-PCR results of IL15RA mRNA in OSRC-2 and ACHN cell lines with METTL3 overexpression. (G-H). MeRIP-PCR results of IL15RA mRNA in OSRC-2 and ACHN cell lines with METTL3 overexpression. (I). SRAMP online tool predicted m6A binding sites. (J). Dual luciferase assay indicated that site 1219 is the m6A binding site of IL15RA mRNA that modulates IL15RA transcription activity in HEK 293 T cell line. (K). RNA decay assay results of IL15RA mRNA in ACHN cell line with METTL3 overexpression. (L-M). Wound closure test results of IL15RA overexpression and silencing in cell lines and quantification statistical bar graph. (N-O). Transwell migration assay results of IL15RA overexpression and silencing in cell lines and quantification statistical bar graph. *P < 0.05; **P < 0.01; ***P < 0.001.
