Fig. 7
From: STAT1 and m6A-mediated IL15RA upregulation promotes metastasis via ZEB1/NF-κΒ axis in ccRCC
IL15RA potentiates metastasis via NF-κΒ/ZEB1 Axis in ccRCC. (A). Western blotting result of p-p65 protein expression in IL15RA overexpression with or without JSH-23. (B). Quantification analysis of IL15RA protein expression in different groups of A. (C). Quantification analysis of p-p65 protein expression in different groups of A. (D). Western blotting result of p-p65 protein expression in IL15RA knockdown with or without NF-κΒ activator-1. (E). Quantification analysis of IL15RA protein expression in different groups of D. (F). Quantification analysis of p-p65 protein expression in different groups of D. (G). Expression of EMT associated genes in IL15RA overexpression condition in OSRC-2 and ACHN cell lines. (H). Vectors including possible binding sites of p-p65 for ZEB1 promoter region were constructed for dual luciferase assays. (I). Results of luciferase assay performed with ZEB1 full promoter region vector and JSH-23 or NF-κΒ activator-1 in both cell lines. (J). Results of luciferase assay performed with different ZEB1 promoter region vector and IL15RA overexpression in both cell lines. (K). The schematic map for IL15RA regulation in ccRCC. *P < 0.05; **P < 0.01; ***P < 0.001.
