Fig. 1
From: A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4
Performance of EHV1-4MP real-time PCR assay in presence of strong competitor. Copy number of isolate shown on x-axis and Ct value shown on y-axis. A) Performance of the EHV1-4MP assay on a serial dilution of EHV-1 isolate in presence of high concentration EHV-4 isolate competitor, B) Performance of the EHV1-4MP assay on a serial dilution of EHV-4 isolate in presence of high concentration EHV-1 isolate competitor, C through E) comparison of Ct values determined by multiplex assay (filled symbols) and singleplex assay (open symbols). Genome copy numbers of EHV-1 and EHV-4 in the serial dilution series were determined by digital PCR.
