Table 1 Equid herpesvirus 1 and 4 multiplex real-time PCR assay primer and probe sequences.
From: A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4
Oligo name | Sequence (5’ – 3’) and probe dyes | Final Concentration | Size |
|---|---|---|---|
EHV-1 genomic real-time PCR assay | |||
EHV1g1775F | ACTCAACCAACAACCCTGGG | 400 nM | 75 bp |
EHV1g1849R | AGAGGAATGGCGGACAACAG | 400 nM | |
EHV1g1801 | CY5-ACACACAGTCTTTTAACACCATGGCAGGC-BHQ3 | 160 nM | |
EHV-1 gB real-time PCR assay | |||
EHV1gB444F | CGTACGCATCGAACCACCTA | 400 nM | 184 bp |
EHV1gB627R | TCTGTCGGACAGGGACGTAT | 400 nM | |
EHV1gB507RC | FAM-CCCCTCCGTGAAGTTTCTCCCAAGGT-BHQ1 | 100 nM | |
EHV-4 gB real-time PCR assay | |||
EHV4F2643 | CCAATAACAACCAGGAGCCTTAA | 500 nM | 82 bp |
EHV4R2724 | CATCGAAGTCGCTAGTGTCATCA | 500 nM | |
EHV4P2676 | TAMRA-AAAGCCTCATACGGCCAAAACGACG-BHQ2 | 200 nM | |
Equid MC1R real-time PCR assay | |||
MC1R2974F | CACCCTCTTCATCGCTTACTACA | 100 nM | 73 bp |
MC1R3046R | CAGCATGGCCACAAAGAAGG | 100 nM | |
MC1R2997p | VIC-ACCACACGGCTGTCCTGCTCTGTC- QSY | 100 nM | |
MS2 real-time PCR assay (46) | |||
MS2-TM-F | TGCTCGCGGATACCCG | 400 nM | 61 bp |
MS2-TM-R | AACTTGCGTTCTCGAGCGAT | 400 nM | |
MS2-Texas Red | TexasRed-ACCTCGGGTTTCCGTCTTGCTCGT-BHQ2 | 120 nM | |
