Table 2 Singleplex assay performance on replicate serial dilutions.
From: A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4
Assay | Slope | Amplification efficiency (%) | Linearity (R2) |
|---|---|---|---|
EHV1g1775 | −3-353 to −3.436 | 95.45 to 98.72 | 0.9970 to 0.9996 |
EHV1gB444 | −3.299 to −3.474 | 94.02 to 101.61 | 0.9981 to 0.9999 |
EHV4gB | −3.231 to −3.479 | 93.84 to 103.94 | 0.9935 to 0.9999 |
EqMC1R | −3.215 to −3.477 | 93.91 to 104.53 | 0.9976 to 0.9996 |
