Table 3 Limit of detection for EHV1-4MP real-time PCR assay.
From: A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4
Assay | Copies in combined PAC1 | Singleplex detection | Multiplex detection |
|---|---|---|---|
EHV1g1775 | 1.98 | 20/20 | 20/20 |
EHV1gB444 | 4.68 | 20/20 | 20/20 |
EHV4gB | 4.14 | 19/20 | 19/20 |