Table 3 Limit of detection for EHV1-4MP real-time PCR assay.

From: A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4

Assay

Copies in combined PAC1

Singleplex detection

Multiplex detection

EHV1g1775

1.98

20/20

20/20

EHV1gB444

4.68

20/20

20/20

EHV4gB

4.14

19/20

19/20

  1. 1Copy number of individual targets in combined PAC was determined by digital PCR.