Table 4 Analytical specificity of EHV1-4MP assay.
From: A multiplex real-time PCR assay for detection of equid herpesvirus 1 and 4
Pathogen | Isolate/strain name | EHV1-4MP assay Ct1 | EHV-1 Ct 47 | EHV-4 Ct 50 | ||
|---|---|---|---|---|---|---|
EHV1g1775 | EHV1gB444 | EHV4gB | ||||
EHV-1 | Ab4 | 15.29 | 15.48 | ND | 16.74 | ND |
EHV-1 | HH1 | 15.12 | 15.51 | ND | 16.64 | ND |
EHV-1 clinical isolate | EHV1-035150 | 14.51 | 14.62 | ND | 16.60 | ND |
EHV-1 clinical isolate | EHV1-014356 | 22.39 | 22.25 | ND | 23.20 | ND |
EHV-2 | H5-57/2018 | ND | ND | ND | ND | ND |
EHV-3 | AR/2007/C3A | ND | ND | ND | ND | 36.60 |
EHV-4 | NS80567 | ND | ND | 18.99 | ND | 17.93 |
EHV-4 clinical isolate | EHV4-049341 | ND | ND | 14.29 | ND | 13.18 |
EHV-4 clinical isolate | EHV4-044856 | ND | ND | 16.87 | ND | 16.51 |
EHV-5 | 2–141/67 | ND | ND | ND | ND | ND |
EHV-8, 104 copies | synthetic | ND | 35.62 | ND | 30 | ND |
EHV-9, 104 copies | synthetic | ND | 34.04 | ND | 28.32 | ND |
Asinine Herpes isolate | 010118 | ND | ND | ND | ND | ND |
Equine Adenovirus 1 | H9NS | ND | ND | ND | ND | ND |
Equine Arteritis Virus | HK116 | ND | ND | ND | ND | ND |
Equine Rhinitis A | Plowright | ND | ND | ND | ND | ND |
Equine Rhinitis B | P1436/71 | ND | ND | ND | ND | ND |
Influenza A | NY-18, H3N8 | ND | ND | ND | ND | ND |
Actinobacillus equuli | AEQU-183,510 | ND | ND | ND | ND | ND |
Actinobacillus lignieresii | ALIG-080448 | ND | ND | ND | ND | ND |
Bordetella bronchiseptica | BB-088661 | ND | ND | ND | ND | ND |
Escherichia coli | ATCC 25,922 | ND | ND | ND | ND | ND |
Klebsiella oxytoca | KO-088499 | ND | ND | ND | ND | ND |
Pasteurella caballi | PCAB-057517 | ND | ND | ND | ND | ND |
Pseudomonas aeruginosa | ATCC 27,853 | ND | ND | ND | ND | ND |
Rhodococcus equi | REQU-280,087 | ND | ND | ND | ND | ND |
Streptococcus dysgalactiae sbsp equisimilis | SDSE-086408 | ND | ND | ND | ND | ND |
Streptococcus equi equi | SEQU-086407 | ND | ND | ND | ND | ND |
Streptococcus zooepidemicus | SZOO-088826 | ND | ND | ND | ND | ND |
Taylorella equigenitalis | NVSL-TE-001 | ND | ND | ND | ND | ND |
