Table 1 Thermal cycling protocol for amplification using different primers.
From: First report and diversity analysis of endophytic fungi associated with Ulva sp. from Iran
Genomic region | Initial denaturation (Temperature// time) | 36 thermal cycles (Temperature//time) | Final extension (Temperature// time) | ||
|---|---|---|---|---|---|
Denaturation | Annealing | Extension | |||
ITS-rDNA | 95 °C// 1 min | 95 °C//1 min | 56 °C// 30 S | 72 °C// 1 min | 72 °C// 5 min |
β-tubulin | 94 °C// 1 min | 94 °C//30 S | 47 °C//30 S | 72 °C//150 S | 72 °C// 10 min |
LSU | 94 °C// 5 min | 94 °C// 60 S | 52 °C// 60 S | 72 °C// 120 S | 72 °C// 10 min |
EF- 1α | 94 °C// 5 min | 94 °C// 15 S | 61 °C// 30 S | 72 °C// 30 S | 72 °C// 8 min |
GAPDH | 95 °C// 5 min | 95 °C//30 S | 57 °C// 30 S | 72 °C// 1 min | 72 °C// 7 min |
