Fig. 4

Metabolite analysis of T. ambiguum under cold stress at different time points: (a) Principal component analysis (PCA) score plot for 12 samples under cold stress treatment; (b) Correlation analysis heatmap showing the similarity between biological replicates in each group (E, F, G, H); (c) Heatmap analysis of metabolic responses across different treatment groups (E, F, G, H) under cold stress.