Fig. 3

Expression dynamics of secreted suppressyn protein in maternal blood (ELISA Analysis). Maternal blood concentrations of secreted suppressyn protein were measured in preeclampsia (PE) patients and matched controls from 29 to 37 weeks of gestation. (a) Changes in the expression of secretory suppressyn (SUPYN) protein in maternal blood throughout pregnancy were assessed in normal pregnancies. (b) Maternal blood concentrations of suppressyn protein were compared between control and PE samples, further subdivided by the presence or absence of fetal growth restriction (FGR) in early-onset PE (EO-PE) and late-onset PE (LO-PE) cohorts. Box plots display the median (horizontal center line), interquartile range (box), mean values (×) and minimum/maximum values (whiskers). The numbers in parentheses indicate the sample sizes used for each group. Statistical significance was evaluated using Mann-Whitney U testing. **p < 0.01; n.s., not significant.