Fig. 2
From: IGF-1 regulates cancer cell immune evasion in prostate cancer
IGF-1 downregulates antigen processing and presentation components. RNA was extracted from DU145, 22Rv1, and Myc-CaP cells 24 h after serum-starving and IGF-treatment as in Fig. 1, and gene expression was assessed by RT-qPCR. Expression of: A. TAP1, TAP2 mRNA in all cell lines and Tapbp in MycCaP; (B) ERAP mRNA, (C) β2M mRNA. D, E. Class I complexes are stabilized at the cell surface by high affinity peptide binding, while peptide-free (empty) heterodimers are generally unstable at the cell surface at physiological temperature. We used Class I antibody to H-2Kq, appropriate to the genotype of FVB mice from which Myc-CaP cells were derived22. Analysis by flow cytometry of H2Kq surface expression in Myc-CaP cells treated with 30 nM IGF-1 or solvent for 2 or 5 days, showing representative flow cytometry plots at 5 days and n = 3 independent experiments. D: percentage H2Kq positivity, E: H2Kq MFI. All graphs represent mean ± SEM of three independent analyses (*p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns, nonsignificant by one-way ANOVA).
