Fig. 4 | Scientific Reports

Fig. 4

From: Advanced phenotyping features utilizing deep learning techniques for automated analysis of stomatal guard cell orientation

Fig. 4

Computational methodology for calculating stomatal opening. This figure illustrates the process used to quantify stomatal opening from segmented images. The approach involves identifying the stomatal pore and surrounding guard cells, calculating their respective areas, and computing the opening ratio as the pore area divided by the guard cell area. This ratio serves as a proxy for the degree of stomatal opening, enabling image-based assessment of stomatal function across large datasets.

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