Fig. 3
Functional Annotation of Ferroptosis Regulatory Networks in DLBCL. A. Bubble plot displaying the results of GO and KEGG enrichment analysis of FRDEGs. The abscissa is GeneRatio, while the ordinate is GO and KEGG terms. Bubble color indicates the adj.p size. B. Network diagram of GO and KEGG enrichment analysis results of FRDEGs. Purple nodes indicate entries, red or blue nodes indicate FRDEGs, and color indicates log2FC value. The lines represent the relationship between the entries and molecules. C. Heatmap of the results of GO and KEGG enrichment analysis of FRDEGs. Circles indicate that the corresponding FRDEGs were enriched under the corresponding entries. The circle color indicates the log2FC value. Circle size indicates the adj.p value. The screening criteria for GO and KEGG enrichment analysis were adj.p < 0.05 and FDR value (q value) < 0.25. The p correction method was Benjamini–Hochberg (BH). D. Landscape visualization of GSEA in TCGA-DLBCL. Each mountain’s position relative to zero encodes NES direction (left = negative, right = positive). The area of a mountain indicates the absolute value of NES; The color of the mountain indicates the size of the adj.p value. E. Heatmap of enrichment scores between Clusters 1 and 2 of GSVA results in TCGA-DLBCL dataset. Blue and red samples represent Clusters 1 and 2, respectively; the Heatmap indicates the enrichment score. GO, Gene Ontology; KEGG, Kyoto Encyclopedia of Genes and Genomes; FRDEG, ferroptosis-related differentially expressed gene; FDR, false discovery rate; GSEA, gene set enrichment analysis; GSVA, gene set variation analysis.
