Fig. 5 | Scientific Reports

Fig. 5

From: Proinflammatory macrophage secretome enhances temozolomide sensitivity in glioblastoma via pSTAT3-mediated downregulation of DNA repair enzymes

Fig. 5

Preferential expression of MGMT and MPG in glioblastoma patient samples with STAT3 activation and associated survival outcomes. (A) Western blot analysis (n = 4) of MGMT and MPG protein levels, as well as STAT3/STAT1 phosphorylation, in A172 and T98G cells and 15 patient-derived tumor samples. Original blots are presented in supplementary material: Figure s7. (B) Survival analysis of a glioblastoma cohort treated with TMZ from the TCGA dataset (334 patients) was conducted using the cBioPortal platform. Patients were classified into two groups based on high versus low expression or activation levels. Kaplan–Meier plots were generated to compare progression-free survival (PFS) among groups with high expression of MGMT, MPG, MGMT + MPG, and pSTAT3. The median mRNA expression levels of DNA repair enzymes or median phosphorylated protein levels were used as cutoffs to define high versus low expression or activation. Statistical significance between survival curves was assessed using the log-rank test.

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