Fig. 6

siRNA targeting of MPG, alone or with MGMT knockdown, potentiates TMZ efficacy. (A) GBM cells were transfected with scrambled (siC) or double siMGMT and siMPG for 48 h, followed by TMZ treatment for 72 h, as shown in the schematic protocol. Expression of repair enzymes was detected by Western blot analysis (representative image from three experiments), with β-actin as a loading control (original blots are presented in supplementary material: Figure s8). (B) The cytotoxic effect of TMZ was assessed by MTT assay in A172 and T98G cells transfected with siRNAs (siC, siMGMT, siMPG, or siMGMT/siMPG) following exposure to TMZ or vehicle. Cell viability was normalized to siC-untreated cells (100%) (mean ± SD, n = 3). Kruskal-Wallis test: *p < 0.05 or **p < 0.01 versus siC-untreated; #p < 0.05 or ##p < 0.01 versus siC/TMZ-treated.