Fig. 6

2DG reverses the effect of LPS on inflammatory gene expression in MxG and MG. MxG were treated with LPS (200 ng/mL) for 24 h followed by vehicle or 2DG (200 µM) for 24 h (a: qRT-PCR and b–f: InCell Western assay). In other experiments, MG were treated with LPS (200 ng/mL) for 24 h then vehicle or 2DG (200 µM) was added for 12 and 24 h followed by staining for inflammatory markers by immunocytochemistry (g–l). LPS, lipopolysaccharide; 2DG, 2-Deoxy-D-glucose; NT, no treatment; MxG, mixed glial cells; MG, microglia; ns, not significant; Arg1, arginase-1. qRT-PCR data are derived from 3 independent experiments, each including a minimum of 3 biological replicates. Quantification of InCell Western assay and IHC is derived from a minimum of 3 independent experiments, each including at least 3 biological replicates. Bar graphs are presented as fold change relative to the NT group in InCell Western assay and are expressed as mean ± SEM. For immunocytochemistry, bar graphs are the number of the cells per field or percentage of double positive cells. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001 (for qRT-PCR, solid asterisks refer to LPS vs. NT; hollow asterisks refer to LPS + 2DG vs. LPS), One way ANOVA with Tukey multiple comparison testing.