Fig. 1
From: Modeling of lung-liver interaction during infection in a human fluidic organ-on-a-chip
Fluidic lung-liver system setup and the stimulation of HBEC and Huh-7. (A) Schematic display of the fluidic system. The tubing connects the lung module, liver module and reservoir supported by a peristaltic Parker PF600 pump. The arrows indicate the direction of the liquid flow, the red part of the silicone tubing has a diameter of 2.4 mm and the green part of 1.6 mm. (B) Representative microscopic image of a confluent and differentiated HBEC culture in ALI-conditions. (C) Development of TEER of HBEC during differentiation from day 0 to day 21 under ALI-conditions. (D) Development of TEER of ATC cultures during differentiation from day 0 to day 7 under ALI-conditions. (E–G) Stimulation of HBEC in the fluidic system with NTHi and the gene expression of (E) TNF-α, (F) IL-1ß, and (G) IL-6 24 h after the stimulation. *p < 0.05, **p < 0.01, n = 3, mean values and error bars of the SEM, unpaired Student´s t-test.
