Table 2 Circulating mediators 24 and 48 h after bacterial stimulation compared to the unstimulated control group quantified by Luminex assays. Data were compared to the control using one-way ANOVA with Tukey post hoc test, n = 3. Significant values are in [bold].
From: Modeling of lung-liver interaction during infection in a human fluidic organ-on-a-chip
Analyt [pg/ml] | 24 h | 48 h | ||||||
|---|---|---|---|---|---|---|---|---|
Li | LiLu | LiLu_NTHi | LiLu_PAO1 | Li | LiLu | LiLu_NTHi | LiLu_PAO1 | |
α2-macro-globulin | 8375,33 ± 896,6 | 7791 ± 1978,87 | 11,552,67 ± 4959,85 | 16,878 ± 10,242 | 16,659,67 ± 1848,13 | 55,247,67 ± 33,501,62 p = 0,0447 | 47,711 ± 15,555,45 | 58,666,33 ± 10,788,39 |
CRP | 19,96 ± 0 | 19,96 ± 0 | 19,96 ± 0 | 19,96 ± 0 | 19,96 ± 0 | 19,96 ± 0 | 19,96 ± 0 | 19,96 ± 0 |
MCP-1 | 12,72 ± 0 | 12,72 ± 0 | 21,5 ± 15,21 | 33,66 ± 36,26 | 12,72 ± 0 | 13,83 ± 1,57 | 16,79 ± 4,09 | 20,24 ± 3,02 p = 0,0385 |
MIP-1α | 191,52 ± 5,3 | 193,01 ± 11,82 | 257,93 ± 19,37 | 260,7 ± 48,4 | 287,64 ± 4,3 | 413,03 ± 121,61 p = 0,004 | 382,2 ± 11,49 | 410,82 ± 17,05 |
IP-10 | 3,33 ± 0,48 | 3,21 ± 1,05 | 4,47 ± 1,09 | 6,32 ± 3,81 | 12,03 ± 3,87 | 60,5 ± 66,99 | 24,7 ± 7,6 | 26,06 ± 5,46 |
IL-1β | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 |
IL-12/IL-23 p40 | 181,23 ± 34,35 | 192,15 ± 52,92 | 276,85 ± 37,28 | 355,13 ± 165,55 | 677,22 ± 153,58 | 2107,26 ± 1989,05 | 1156,01 ± 148,61 | 1191,33 ± 180,48 |
IL-1ra | 9,18 ± 1,47 | 110,39 ± 94,15 | 92,88 ± 61,14 | 129,89 ± 49,72 | 26,03 ± 8,71 | 276,18 ± 55,23 | 265,98 ± 185,79 | 207,76 ± 165,07 |
IL-6 | 0,87 ± 0 | 0,87 ± 0 | 2,13 ± 2,18 | 1,8 ± 1,62 | 0,87 ± 0 | 0,9 ± 0,04 | 1,76 ± 1,54 | 2,11 ± 1,95 |
TNF-α | 1,27 ± 0 | 1,27 ± 0 | 1,31 ± 0,07 | 1,56 ± 0,5 | 1,27 ± 0 | 1,43 ± 0,23 | 1,47 ± 0,26 | 2,1 ± 0,44 p = 0,0308 |
BDNF | 0,88 ± 0 | 0,88 ± 0 | 0,88 ± 0 | 0,88 ± 0 | 0,88 ± 0 | 0,88 ± 0 | 0,88 ± 0 | 0,88 ± 0 |
CD31 | 148,88 ± 0 | 148,88 ± 0 | 148,88 ± 0 | 148,88 ± 0 | 148,88 ± 0 | 148,88 ± 0 | 148,88 ± 0 | 148,88 ± 0 |
Eotaxin | 0,18 ± 0 | 0,18 ± 0 | 0,18 ± 0 | 0,18 ± 0 | 0,18 ± 0 | 0,18 ± 0 | 0,18 ± 0 | 0,18 ± 0 |
IFNγ | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 | 3,2 ± 0 |
Myoglobin | 249,91 ± 0 | 249,91 ± 0 | 249,91 ± 0 | 249,91 ± 0 | 249,91 ± 0 | 249,91 ± 0 | 249,91 ± 0 | 249,91 ± 0 |
NTpro-BNP | 1,67 ± 0 | 1,67 ± 0 | 1,67 ± 0 | 1,67 ± 0 | 1,67 ± 0 | 1,67 ± 0 | 1,67 ± 0 | 1,86 ± 0,33 |
PAI-1 | 447,45 ± 117,15 | 1547,83 ± 956,51 | 1335,81 ± 827,52 | 654,9 ± 375,21 | 900,16 ± 244,22 | 3623,38 ± 3453,55 | 3090 ± 352,49 | 2186,21 ± 2129,8 |
S100A8/A9 | 20,38 ± 0 | 248,43 ± 388,81 | 59,01 ± 66,91 | 47,35 ± 16,95 | 79,65 ± 75,1 | 328,88 ± 315,32 | 340,4 ± 276,05 | 5321,02 ± 9178,98 |
SCF | 0,24 ± 0 | 0,24 ± 0 | 0,24 ± 0 | 0,24 ± 0 | 0,24 ± 0 | 0,24 ± 0 | 0,24 ± 0 | 0,37 ± 0,23 |
Angio-poietin-2 | 41,31 ± 5,23 | 41,35 ± 9,41 | 83,31 ± 27,42 | 86,93 ± 59,31 | 72,22 ± 14,1 | 100,82 ± 34,07 | 128,69 ± 33,64 | 215,02 ± 85,86 p = 0,0158 |
IL-10 | 1,65 ± 0 | 1,65 ± 0 | 1,65 ± 0 | 1,65 ± 0 | 1,65 ± 0 | 2,1 ± 0,64 | 2,04 ± 0,33 | 2,47 ± 1,11 |
IL-18 | 5,62 ± 2,43 | 3,74 ± 0,9 | 8,42 ± 5,48 | 8,14 ± 1,93 | 10,09 ± 1,76 | 9,81 ± 0,8 | 12,64 ± 1,96 | 14,92 ± 5,23 |
IL-8/ CXCL8 | 345,35 ± 157,36 | 385,93 ± 129,5 | 845,43 ± 634,95 | 1085,65 ± 746,58 | 884,07 ± 232,48 | 1029,2 ± 302,77 | 1845,33 ± 670,6 | 2410 ± 122,05 p = 0,031 |
S100A9 | 6,11 ± 2,13 | 14,58 ± 6,89 | 12,53 ± 5,58 | 12,83 ± 3,87 | 15,95 ± 8,7 | 23,96 ± 10,92 | 31,99 ± 7,18 | 3154,02 ± 5423,03 |
VEGF | 90,19 ± 8,92 | 132,14 ± 26,98 | 222,62 ± 62,19 | 154,32 ± 45,14 | 242,33 ± 32,03 | 622,08 ± 311,16 | 492,48 ± 101,46 | 724,35 ± 526,36 |
