Fig. 1

L. niger differential expression and functional enrichment of spermathecal proteins. (A) 720 (shown) of 1447 proteins quantified were differentially expressed (5% FDR, Benjamini-Hochberg correction). Black tiles indicate missing values. 274 (B), 487 (C), and 485 (D) proteins were differentially expressed in the virgin-to-incipient transition, the virgin-to-established transition, and the incipient-to-established transition, respectively. Select protein functions of interest are color-coded. FC = fold-change; INDY = protein “i m not dead yet”. Structural components of sperm flagella are categorized as “putative sperm proteins.” The horizontal dotted lines indicate the 5% FDR threshold, and the vertical dotted lines indicate log₂(FC) = 2. Proteins with adjusted p < 0.05 and log(FC) > 2 are considered intensely significant. E) Significantly enriched GO terms (shown; 5% FDR, Benjamini-Hochberg correction) were only identified among up-regulated proteins in one contrast, the virgin-to-incipient transition. Higher multifunctionality scores indicate that enrichments are more likely to occur by chance due to component proteins being tied to many functions.