Table 2 Optical density (OD) of Bacillus subtilis before and after various treatments. Initial OD values were recorded before treatment, and final OD mean values were measured post-treatment for control, acidic (pH 4), alkaline (pH 9), hydrogen peroxide (H₂O₂), heat (42 °C), cold (refrigeration temperature), and ethanol (5% w/v).
Group type | Initial OD | Final OD (mean values) (after 2 h treatment) |
|---|---|---|
Control | 0.63 | 1 |
Acidic | 0.63 | 1 |
Alkaline | 0.63 | 1 |
H202 | 0.63 | 0.48 |
Heat | 0.63 | 0.625 |
Cold | 0.63 | 0.645 |
Ethanol | 0.63 | 0.72 |
