Fig. 9
From: Neutrons reveal the dynamics of leaf thylakoids in living plants
Duckweed sample preparation. Left-side panels show representative batches during growing and harvesting phase; Middle and right-side panels show the two NSE samples collected in quartz cells and mounted on the NSE sample holder frame together with the D2O solvent sample. Note that the NSE samples were interchanged in the middle of the NSE experiment (after ~ 65 h) to ensure fresh living plants for the entire length of the measurement. Two identical samples were prepared consecutively and swapped during NSE measurements to make sure the plants are alive. The samples were visually inspected at regular intervals (daily) for signs of wilting and leaves breakage. When those slightly occurred, after approximatively 65 h, the first sample was retrieved and replaced with the second sample to ensure fresh and potent plants, see Fig. 9. No major wilting signs or sample decomposition was observed for the duration of the experiment (130 h). Please see the Supporting Information, Figure S4, for additional bench-top testing of the viability of the samples after extended soaking time in D2O.
