Fig. 4 | Scientific Reports

Fig. 4

From: Integration of Single-cell and bulk RNA sequencing data uncovers lymphatic metastasis-related prognostic genes and a predictive model in bladder cancer

Fig. 4

Screening of prognostic genes. A: Volcano plot of DEGs obtained by differential analysis between BLCA and control samples in the training set. B: Heatmap of DEGs. C: Intersection of DEGs and characteristic genes of epithelial cell cluster 3, resulting in 58 intersecting genes. D: Univariate Cox analysis of intersecting genes. E: A screening model was constructed using 10 machine learning algorithms and 100 algorithm combinations based on the expression levels of candidate genes in BLCA samples and clinical features (time and status). The model with the highest average concordance index (C-index) in both the training and validation sets was selected, and the genes within it were extracted as prognostic genes. F: Expression levels of prognostic genes in BLCA and normal samples. * P < 0.05; **, P < 0.01; ***, P < 0.001.

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