Fig. 2

Pancreatic epithelial ELP3 inactivation in the pancreas and acute pancreatitis. (A) Schematic representation of Elp3 inactivation in pancreatic epithelial cells (PEC) using a floxed Elp3 exon 2 (Elp3-E2) allele and Pdx1-Cre driven deletion. (B) Representative pancreas images from Elp3^WT and Elp3^∆PEC mice with or without caerulein treatment. Scale bar: 5 mm. (C) Histological staining (hematoxylin & eosin) of pancreatic sections from Elp3^WT and Elp3^∆PEC mice with or without caerulein treatment. Scale bar: 50 μm. (D-E) Immunofluorescence of F4/80 & E-cadherin (D) and vimentin & E-cadherin (E), to label macrophages (F4/80), fibroblasts (vimentin), and epithelial cells (E-cadherin), with Hoechst nuclear counterstaining, in pancreatic sections from Elp3^WT and Elp3^∆PEC mice with or without caerulein treatment. Scale bar: 50 μm.