Fig. 2
From: The role of thrombospondin-1 in trehalose-induced autophagy and ocular hypertension in mice
Trehalose increased LC3-II protein expression and autophagy-associated genes expression in hTM cells. Cultured hTM cells were treated with 100 mM trehalose and vehicle controls in serum-free medium for 48 h. Protein expression of LC3-II was analyzed by western blot. (a) Representative of three individual western blots of LC3-II expression in hTM cells. The original blot is presented in Supplementary Fig. S4. (b) The ratio of LC3-II: LC3-I protein level (n = 3 different cell strains) as shown in (a). The gene expression levels of (c) ATG5 (n = 3 different cell strains), (d) ATG7 (n = 3 different cell strains), (e) NFE2L2 (n = 4 different cell strains), and (f) SQSTM1 (n = 4 different cell strains) were analysed by RT-qPCR. Data represent mean ± SEM. Statistical significance (unpaired Student’s t-test) relative to vehicle control is indicated by *(P < 0.05) and **(P < 0.01). Tre: trehalose; C: vehicle control.
