Fig. 4

Different cellular stressors, although not serum starvation, activated eMI-sensor puncta formation in RTH-149 cells. (A) Representative images of RTH-149 cells expressing the eMI-reporter and incubated for 16 h with either CT medium, H₂O₂ (25 µM), glucose (25 mM, High-Glucose), etoposide (50 µM), a medium lacking serum (-FBS) alone, or a medium lacking both serum and amino acids (-FBS –AA). (B) Quantification of KFERQ-Venus number of puncta per cell. All values correspond to individual images (CT 80; H₂O₂ 86; High-Glucose 76; Etoposide 86; –FBS 87; –FBS –AA 76), with > 15 images/experiment in a total of 3 independent experiments (> 600 cells per condition). Differences between two groups were assessed using the non-parametric Mann Whitney test, and the p values are indicated in the figure. All data are presented as Mean ± SEM; scale bars: 10 µm.