Fig. 6
From: Quantifying cancer- and drug-induced changes in Shannon information capacity of RTK signaling
In silico protocol optimization and channel capacity estimation. In panels A–E, colors correspond to different cell lines and conditions, as indicated in the common legend. All 0.3 – 3 µM ALKi concentrations were pooled. (A) Population-average ERK-KTR response to a light pulse preceded by a long interval (25 min). (B) Average response amplitude measured 7 min after pulse, as a function of the interval preceding the light pulse. (C) Same as B but measured 2 min after the pulse. (D) The MLP classifier’s logit Bayesian update to the prior pulse probability, as a function of the interval preceding the light pulse. (E) Optimized input interval distribution. The black line shows the interval distribution used in the experimental protocol. (F) Channel capacity (i.e., bitrate achievable with the optimized input interval distribution) in transmitting cells, compared to the experimentally determined bitrates reported in Fig. 3C. Data from all experimental replicates for a given cell line and condition were pooled and analyzed jointly. Panels D–F plotted based on transmitting cells only.
