Fig. 3
Effects of B1 asRNA on the expression of senescence related genes. (A) Effect of B1 asRNA treatment on the mRNA expression levels of the p53 gene. Compared with the PHA group, the expression of p53 mRNA in the B1 asRNA group was significantly down-regulated at 48 h of treatment (mean ±SD of three independent experiments). **P < 0.01. (B) B1 asRNA down-regulated the expression level of p16Ink4a gene at 48 h after treatment (mean ±SD of three independent experiments).* P < 0.05. (C) B1 asRNA down-regulated the expression level of p21 gene at 48 h after treatment (mean ±SD of three independent experiments).* P < 0.05. (D) Effect of B1 asRNA treatment on the mRNA expression levels of the Lamin B1 gene. Lamin B1 mRNA expression was significantly up-regulated at 24 h, 48 h and 72 h of treatment (mean ± SD of three independent experiments).* P < 0.05, ** P < 0.01, *** P < 0.001. (E) Effect of B1 asRNA treatment on the mRNA expression levels of the H2AX gene. Compared with the PHA group, the expression of H2AX mRNA was significantly up-regulated at 48 h and 72 h of treatment (mean ±SD of three independent experiments). *: P < 0.05. (F) B1 asRNA down-regulated the protein level of γ-H2AX at 48 h after treatment. (G) Representative image of γ-H2AX protein detection by IF (magnification 25 × 10). (H) Effect of B1 asRNA treatment on the mRNA expression levels of the SASP genes. The mRNA expression levels were significantly down-regulated at 48 h after B1 asRNA treatment (mean ±SD of three independent experiments). *P < 0.05.
