Fig. 4

The effects of B1 asRNA on the expression of transcription factor genes. RT-qPCR was used to detect the mRNA expression of Nanog, Oct4, Sox2, Klf4, c-Myc and c-FOS in lymphocytes of PHA group and B1 asRNA group at 24 h, 48 h and 72 h in vitro. The mRNA expressions of Nanog (A), Oct4 (D), Sox2 (G), Klf4 (J), c-Myc (M) and c-Fos (P) were up-regulated 24 h, 48 h and 72 h after B1 asRNA treatment (mean ± SD of three independent experiments). *P < 0.05, **P < 0.01, ***P < 0.001. Western blotting was used to detect the protein expression levels of Nanog, Oct4, Sox2, Klf4, c-Myc and c-FOS in lymphocytes of PHA group and B1 asRNA group at 24 h, 48 h and 72 h in vitro. The protein expression levels of Nanog (B–C), Oct4 (E–F), Sox2 (H, I), Klf4 (K, L), c-Myc (N, O) and c-Fos (Q, R) were up-regulated after B1 asRNA treatment. The protein relative expression levels were means ±SD of three independent experiments). *P < 0.05, **P < 0.01.